Electrochemical methods have gained significant attention for the rapid, sensitive, and cost-effective detection of plant viruses, which are a major threat to global agriculture. These techniques combine electrochemical transducers with biological recognition elements such as antibodies, aptamers, and nucleic acids to detect plant virus particles or their genetic material with high specificity. Advances in nanotechnology, including the use of materials like graphene, gold nanoparticles, and carbon nanotubes, have significantly enhanced the sensitivity and selectivity of electrochemical biosensors. Commonly used techniques such as amperometry, voltammetry, and electrochemical impedance spectroscopy enable real-time detection, even at very low concentrations of viral biomarkers. These biosensors offer numerous advantages over traditional detection methods, including portability, speed, and the potential for point-of-care applications in the field. This chapter explains recent developments in electrochemical biosensors for plant virus detection, focusing on the integration of nanomaterials, sensor design, and their application in diagnosing economically important plant viruses. It also discusses the challenges in scaling up these technologies for widespread agricultural use and explore future prospects for improving detection efficiency through the development of hybrid systems and advanced sensing platforms. Electrochemical biosensors present a promising tool for enhancing agricultural sustainability and reducing the impact of viral diseases on crop yields. In this chapter, the main focus is upon various electrochemical methods of virus detection using advance tools and techniques.