Scholarlink

The DNA extraction from plant viruses begins with the collection of plant samples that exhibit viral infection symptoms. The tissue is then crushed into fine powder using liquid nitrogen. The next step is to lyse cell membranes and release the viral DNA using the extraction buffer, composed of cetyltrimethylammonium bromide (CTAB) and other reagents. CTAB protocol is the precise and efficient technique for DNA extraction from plant viruses for understanding plant–virus interactions and developing effective diagnostic tools. Subsequent steps involve chloroform:isoamyl-alcohol extractions to remove proteins, polysaccharides, and other contaminants. The DNA is precipitated with isopropanol, washed with ethanol, and finally resuspended in TE buffer or distilled water. The resulting DNA can be quantified using a spectrophotometer or fluorometer, measuring the yield and purity of the extracted material. This optimized CTAB-based protocol offers several advantages, including simplicity, cost-effectiveness, and reproducibility. It is adaptable to a wide range of plant species, making it a valuable tool for researchers and diagnosticians studying plant virology.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Extraction of High-Quality Genomic DNA from Plants Using Modified CTAB-Based Method

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Andleeb Javaid,
  • Sumiah Wani,
  • Nulevino Iralu,
  • Lubna Javaid,
  • Dasari Meghanath,
  • Gowhar Ali,
  • Aflaq Hamid

摘要

The DNA extraction from plant viruses begins with the collection of plant samples that exhibit viral infection symptoms. The tissue is then crushed into fine powder using liquid nitrogen. The next step is to lyse cell membranes and release the viral DNA using the extraction buffer, composed of cetyltrimethylammonium bromide (CTAB) and other reagents. CTAB protocol is the precise and efficient technique for DNA extraction from plant viruses for understanding plant–virus interactions and developing effective diagnostic tools. Subsequent steps involve chloroform:isoamyl-alcohol extractions to remove proteins, polysaccharides, and other contaminants. The DNA is precipitated with isopropanol, washed with ethanol, and finally resuspended in TE buffer or distilled water. The resulting DNA can be quantified using a spectrophotometer or fluorometer, measuring the yield and purity of the extracted material. This optimized CTAB-based protocol offers several advantages, including simplicity, cost-effectiveness, and reproducibility. It is adaptable to a wide range of plant species, making it a valuable tool for researchers and diagnosticians studying plant virology.