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Flow cytometry enables the detection and characterization of specific cell populations, and proper identification of particular cell types can be assessed by immunohistochemistry. Here, we describe the identification and sorting of nonhematopoietic stem cells expressing stem cell antigen-1 from adult murine retinas using flow cytometry and immunohistochemistry strategies. These sorted cells can be further used for regenerative purposes.

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Nonhematopoietic Stem Cell Identification and Sorting in Adult Retina

  • 实验方案

  • 发表日期：

    2025年

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  • Ana Flores,
  • Laura Fernández-Sánchez,
  • Oksana Kutsyr,
  • Alberto Yáñez,
  • María Luisa Gil,
  • Daniel Gozalbo,
  • Victoria Maneu,
  • Pedro Lax

摘要

Flow cytometry enables the detection and characterization of specific cell populations, and proper identification of particular cell types can be assessed by immunohistochemistry. Here, we describe the identification and sorting of nonhematopoietic stem cells expressing stem cell antigen-1 from adult murine retinas using flow cytometry and immunohistochemistry strategies. These sorted cells can be further used for regenerative purposes.