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To isolate toxin-producing Gram-positive bacteria from food sample, one may enrich the food sample in liquid medium containing potassium tellurite, sodium azide, or thallium acetate (at concentrations of 0.1–0.5 g/L) for minimum 48 to 72 h. After incubation, isolate the bacteria on selective media for targeted Gram-positive bacterial genus.

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Detection of Gram-Positive Bacterial Toxin from Food Sample by Ouchterlony Double Diffusion Immunoassay Method

  • 章节

  • 发表日期：

    2025年

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  • Leena Sheth,
  • Abhijeet Joshi

摘要

To isolate toxin-producing Gram-positive bacteria from food sample, one may enrich the food sample in liquid medium containing potassium tellurite, sodium azide, or thallium acetate (at concentrations of 0.1–0.5 g/L) for minimum 48 to 72 h. After incubation, isolate the bacteria on selective media for targeted Gram-positive bacterial genus.