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This study explores the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detecting Gram-positive bacterial toxins in food. Focusing on toxins like staphylococcal enterotoxins and Clostridium botulinum neurotoxins, the research optimizes sample preparation techniques for various food matrices. LC-MS/MS, known for high sensitivity and specificity, is employed for its ability to identify multiple toxins concurrently. The method demonstrates excellent sensitivity, surpassing regulatory thresholds, and proves reliable in complex food samples. The study emphasizes the significance of advanced analytical techniques for efficient screening, aiding in early intervention and prevention of foodborne outbreaks.

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Detection of Foodborne Gram-Positive Bacterial Toxins by Liquid Chromatography-Tandem Mass Spectrometry

  • 章节

  • 发表日期：

    2025年

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  • Sanchita Choubey

摘要

This study explores the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detecting Gram-positive bacterial toxins in food. Focusing on toxins like staphylococcal enterotoxins and Clostridium botulinum neurotoxins, the research optimizes sample preparation techniques for various food matrices. LC-MS/MS, known for high sensitivity and specificity, is employed for its ability to identify multiple toxins concurrently. The method demonstrates excellent sensitivity, surpassing regulatory thresholds, and proves reliable in complex food samples. The study emphasizes the significance of advanced analytical techniques for efficient screening, aiding in early intervention and prevention of foodborne outbreaks.