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We describe a protocol to amplify DNA barcodes of known and unknown taxa of Phytophthora and related plant pathogenic oomycetes from a range of environments. The methods focus on sampling pathogen propagules from water using in situ sampling and filtration equipment and buffers that enable efficient storage and DNA extraction for later downstream processing.

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The Use of eDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples

  • 实验方案

  • 发表日期：

    2025年

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  • Eva Randall,
  • Beatrix Keillor,
  • David E. L. Cooke

摘要

We describe a protocol to amplify DNA barcodes of known and unknown taxa of Phytophthora and related plant pathogenic oomycetes from a range of environments. The methods focus on sampling pathogen propagules from water using in situ sampling and filtration equipment and buffers that enable efficient storage and DNA extraction for later downstream processing.