Two-photon microscopy has gained widespread recognition as a potent tool for providing both qualitative and quantitative information in the field of bone research. This chapter outlines a detailed protocol for using two-photon microscopy to track the colonization of cancer cells to bone, using frozen bone samples derived from xenograft mouse models.

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Ex Vivo Analysis of Single Cancer Cell Homing to Murine Bone via Two-Photon Microscopy

  • Gloria Allocca,
  • Ning Wang

摘要

Two-photon microscopy has gained widespread recognition as a potent tool for providing both qualitative and quantitative information in the field of bone research. This chapter outlines a detailed protocol for using two-photon microscopy to track the colonization of cancer cells to bone, using frozen bone samples derived from xenograft mouse models.