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Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays: electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay—five commonly used methods for studying and altering gene transcription.

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Analysis of Transcriptional Regulation in Bone Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Huilin Jin,
  • Antonia Sophocleous,
  • Asim Azfer,
  • Stuart H. Ralston

摘要

Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays: electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay—five commonly used methods for studying and altering gene transcription.