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Measuring respiration rate can be a powerful way to assess energetic function in isolated mitochondria and intact cells. Current plate-based methods have several advantages over older suspension-based systems, including greater throughput and the requirement of only microgram quantities of material. In this chapter, we provide an update to our previously published methods for plate-based measurement of oxygen consumption in isolated adherent mitochondria in a 96-well format plate. We also describe methods for performing the same measurements on intact cells in a 96-well format.

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Plate-Based Measurement of Respiration by Isolated Mitochondria and by Intact Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Shona A. Mookerjee

摘要

Measuring respiration rate can be a powerful way to assess energetic function in isolated mitochondria and intact cells. Current plate-based methods have several advantages over older suspension-based systems, including greater throughput and the requirement of only microgram quantities of material. In this chapter, we provide an update to our previously published methods for plate-based measurement of oxygen consumption in isolated adherent mitochondria in a 96-well format plate. We also describe methods for performing the same measurements on intact cells in a 96-well format.