Scholarlink

Vesicular stomatitis virus (VSV)-based vaccination has shown protective efficacy against filovirus infection. Following the approval of a VSV-based vaccine against Ebola virus, there have been efforts toward applying the same platform for other filoviruses, including Marburg virus (MARV) and Sudan virus. Because these vaccines express filovirus glycoproteins, they are also a valuable tool to study filovirus entry under biosafety level 2 conditions. In the protocol described below, we outline how to genetically manipulate a full-length VSV vector by removing the native VSV glycoprotein and replacing it with the surface-expressed MARV glycoprotein. In addition, we describe the recovery procedure of these recombinant, full-length VSVs and detail the necessary steps of virus propagation.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Generation, Recovery, and Propagation of a Recombinant Vesicular Stomatitis Virus Expressing the Marburg Virus Glycoprotein

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Hanna Anhalt,
  • Andrea Marzi

摘要

Vesicular stomatitis virus (VSV)-based vaccination has shown protective efficacy against filovirus infection. Following the approval of a VSV-based vaccine against Ebola virus, there have been efforts toward applying the same platform for other filoviruses, including Marburg virus (MARV) and Sudan virus. Because these vaccines express filovirus glycoproteins, they are also a valuable tool to study filovirus entry under biosafety level 2 conditions. In the protocol described below, we outline how to genetically manipulate a full-length VSV vector by removing the native VSV glycoprotein and replacing it with the surface-expressed MARV glycoprotein. In addition, we describe the recovery procedure of these recombinant, full-length VSVs and detail the necessary steps of virus propagation.