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Multiplex immunoassays allow for the simultaneous detection of antibody populations targeting multiple antigens in a very sensitive, high-throughput format. This assay has applications in seroprevalence, serodiagnosis, and vaccine assessment with greater sensitivity and dynamic range than standard ELISAs (enzyme-linked immunosorbent assays). Here, we show in detail the methods for multiplex immunoassays to detect IgG to the Marburg virus glycoprotein.

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Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein Using a Multiplex Immunoassay Platform

  • 实验方案

  • 发表日期：

    2025年

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  • Aquena Ball,
  • Teri Ann S. Wong,
  • Axel T. Lehrer

摘要

Multiplex immunoassays allow for the simultaneous detection of antibody populations targeting multiple antigens in a very sensitive, high-throughput format. This assay has applications in seroprevalence, serodiagnosis, and vaccine assessment with greater sensitivity and dynamic range than standard ELISAs (enzyme-linked immunosorbent assays). Here, we show in detail the methods for multiplex immunoassays to detect IgG to the Marburg virus glycoprotein.