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Time-lapse microscopy is a valuable, widely employed technique for investigating cell cycle dynamics. This chapter will guide you in using this tool to determine the duration of anaphase as a measure of mitotic exit kinetics in budding yeast cells. The methodology explained here is based on monitoring the mitotic spindle and provides a comprehensive guide covering all aspects, from sample preparation and microscopy to data analysis.

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Determination of Anaphase Duration by Time-Lapse Microscopy in Budding Yeast

  • 实验方案

  • 发表日期：

    2025年

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  • Mariam Huda,
  • Ayse Koca Caydasi

摘要

Time-lapse microscopy is a valuable, widely employed technique for investigating cell cycle dynamics. This chapter will guide you in using this tool to determine the duration of anaphase as a measure of mitotic exit kinetics in budding yeast cells. The methodology explained here is based on monitoring the mitotic spindle and provides a comprehensive guide covering all aspects, from sample preparation and microscopy to data analysis.