Scholarlink

The mitotic spindle consists of aligned filaments of dynamic microtubules that faithfully segregate mitotic chromosomes. This view of the mitotic spindle was initially established by detecting weak birefringence of the aligned filaments, which was realized by developing polarized light microscopy (Inoué S, Chromosoma 5:487–500. https://doi.org/10.1007/bf01271498 , 1953). Inoué et al. developed a centrifuge polarizing microscope (CPM) (Inoué S et al., J Microsc 201:341–356. https://doi.org/10.1046/j.1365-2818.2001.00850.x , 2001; Inoué S et al., Centrifuge microscope capable of realizing polarized light observation (US Patent: US1907803A), 1999) to further understand the structural and physical basis of the functional positioning of centrosome-based structures (including the mitotic spindle) inside living cells. This chapter describes the procedure for observing biological specimens using CPM by focusing on Caenorhabditis elegans embryos.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Centrifuge Polarizing Microscope (CPM) That Enables the Visualization of Intracellular Structures Under High Centrifugal Forces

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Makoto Goda,
  • Akatsuki Kimura

摘要

The mitotic spindle consists of aligned filaments of dynamic microtubules that faithfully segregate mitotic chromosomes. This view of the mitotic spindle was initially established by detecting weak birefringence of the aligned filaments, which was realized by developing polarized light microscopy (Inoué S, Chromosoma 5:487–500. https://doi.org/10.1007/bf01271498 , 1953). Inoué et al. developed a centrifuge polarizing microscope (CPM) (Inoué S et al., J Microsc 201:341–356. https://doi.org/10.1046/j.1365-2818.2001.00850.x , 2001; Inoué S et al., Centrifuge microscope capable of realizing polarized light observation (US Patent: US1907803A), 1999) to further understand the structural and physical basis of the functional positioning of centrosome-based structures (including the mitotic spindle) inside living cells. This chapter describes the procedure for observing biological specimens using CPM by focusing on Caenorhabditis elegans embryos.