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Microalgae have become a novel, fascinating, and profitable platform for the synthesis of various biomolecules. These biomolecules include proteins, lipids, and carotenoids, which are already approved for commercial applications. The main goal of the developmental process is to enhance the biomolecule yield and microalgal biomass multiplication rate. Over the past few decades, there has been a significant advancement in the technology of microalgae and cyanobacterial genetic engineering. The results of the studies have contributed an elevation in the commercial viability of microalgal products. We describe a protocol to genetically modify the genome of the freshwater microalgae Chlorella vulgaris which possess abundant protein content transformation with a viral vector for the biopharmaceutical manifestation with the help of human basic fibroblast growth factor (bFGF) & SARS-CoV-2 receptor binding domain (SARS-CoV-2 RBD).

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Genome Engineering of Chlorella sp.

  • 章节

  • 发表日期：

    2025年

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  • Fayaazuddin Thajuddin,
  • Chandru Ashok,
  • Thajuddin Nooruddin,
  • Dhanasekaran Dharumadurai

摘要

Microalgae have become a novel, fascinating, and profitable platform for the synthesis of various biomolecules. These biomolecules include proteins, lipids, and carotenoids, which are already approved for commercial applications. The main goal of the developmental process is to enhance the biomolecule yield and microalgal biomass multiplication rate. Over the past few decades, there has been a significant advancement in the technology of microalgae and cyanobacterial genetic engineering. The results of the studies have contributed an elevation in the commercial viability of microalgal products. We describe a protocol to genetically modify the genome of the freshwater microalgae Chlorella vulgaris which possess abundant protein content transformation with a viral vector for the biopharmaceutical manifestation with the help of human basic fibroblast growth factor (bFGF) & SARS-CoV-2 receptor binding domain (SARS-CoV-2 RBD).