Scholarlink

Bacterial tagging with genes encoding for fluorescent proteins is a useful tool to visualize the plant colonization by administered plant growth promotion bacteria using fluorescence microscopy. A stable genetic transformation of the selected strains is preferable for the assessment of their colonization and establishment in the soil or in the plant tissues during long-term, in vivo plant-microbiome engineering experiments. In this chapter, we describe the procedure of conjugation for stable bacteria transformation by using the mini-Tn7 transposon system, an efficient way to genetically manipulate a broad range of Gram-negative bacteria that allows the tagging with a single-copy, stable insertion of the marker gene in the genome.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Conjugation Techniques for Engineering Microbiome Bacterial Species

  • 章节

  • 发表日期：

    2025年

查看全文
  • Lorenzo Vergani,
  • Francesco Riva

摘要

Bacterial tagging with genes encoding for fluorescent proteins is a useful tool to visualize the plant colonization by administered plant growth promotion bacteria using fluorescence microscopy. A stable genetic transformation of the selected strains is preferable for the assessment of their colonization and establishment in the soil or in the plant tissues during long-term, in vivo plant-microbiome engineering experiments. In this chapter, we describe the procedure of conjugation for stable bacteria transformation by using the mini-Tn7 transposon system, an efficient way to genetically manipulate a broad range of Gram-negative bacteria that allows the tagging with a single-copy, stable insertion of the marker gene in the genome.