In rice fields, plant growth-promoting microalgae can be monitored for their dynamics using quantitative PCR (qPCR), a technique that is explored in this study. As the demand for sustainable agricultural practices continues to rise, understanding the intricate relationships between microorganisms and plants becomes crucial. Plant growth-promoting microalgae have gained attention for their potential to enhance crop productivity through various mechanisms, including nutrient cycling and stress tolerance. In this research, we employed qPCR techniques to quantify the abundance of specific microalgal strains known for their plant growth-promoting capabilities. The study involved the collection of soil and rhizosphere samples from rice fields at different growth stages. Through the use of species-specific primers, the qPCR analysis enabled accurate quantification of targeted microalgal populations. Results revealed dynamic shifts in microalgal abundance throughout the rice growth cycle, shedding light on the temporal dynamics of these beneficial microorganisms. The findings contribute valuable insights into the potential use of qPCR as a reliable method for real-time monitoring of plant growth-promoting microalgae in agricultural ecosystems. This research holds promise for optimizing agricultural practices by harnessing the beneficial interactions between microalgae and crops for sustainable and enhanced crop production.