Actinobacteria are widely recognized as prolific producers of secondary metabolites, holding significant agricultural and pharmaceutical importance. Streptomyces, a prominent member of the phylum, is ubiquitous in nature and plays a crucial ecological role, particularly beneficial to plants by harboring traits that promote growth and acting as biocontrol agents. One of the primary ecological roles attributed to Streptomyces is the production of antibacterial and antifungal compounds, crucial for establishing disease-suppressive soil and promoting overall plant health. Moreover, the taxon has been reported to exhibit plant growth-promoting traits, including nutrient provision and tolerance against abiotic stresses. The advancement of next-generation sequencing technology has made it easier for us to explore the full potential of microorganisms distributed across the world. This is especially advantageous in the study of the biosynthetic gene clusters of Streptomyces, as a significant number of them have been considered silent. In this chapter, we outline the protocol for extracting genomic DNA from Streptomyces and subsequently perform genome sequencing to uncover the complete potential of Streptomyces, with particular importance for the agricultural sector.