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The contemporary molecular tools adopted for identifying microalgae include techniques such as quantitative real-time PCR (qPCR), biosensors, multilocus sequence typing (MLST), and microarray-based detection. However, 16S rRNA and 18S rRNA gene sequencing is the most effective method for identifying microorganisms. In recent years, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has gained popularity for microbiological diagnosis and identification, due to its rapid, sensitive, and cost-efficient technique in terms of both labor and materials involved. Microbes are identified based on their protein profiles produced by desorption of either whole cells or cell extracts of an organism, which has resulted in a distinct mass spectrometric database that aids in species-level identification.

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Rapid Detection of Plant-Associated Microalgae by MALDI-TOF Mass Spectrometry

  • 章节

  • 发表日期：

    2025年

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  • K. Shrika,
  • Dhanasekaran Dharumadurai

摘要

The contemporary molecular tools adopted for identifying microalgae include techniques such as quantitative real-time PCR (qPCR), biosensors, multilocus sequence typing (MLST), and microarray-based detection. However, 16S rRNA and 18S rRNA gene sequencing is the most effective method for identifying microorganisms. In recent years, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has gained popularity for microbiological diagnosis and identification, due to its rapid, sensitive, and cost-efficient technique in terms of both labor and materials involved. Microbes are identified based on their protein profiles produced by desorption of either whole cells or cell extracts of an organism, which has resulted in a distinct mass spectrometric database that aids in species-level identification.