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This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET). Focusing on the eBRET2 and NanoBRET™ variants, the chapter covers steps from cell culture to transfection, ligand stimulation, and BRET measurements, offering a robust protocol to examine the temporal aspects of Gq signaling in live cells. This methodology facilitates a nuanced understanding of Gq-mediated cellular responses, but also provides researchers with a valuable tool for dissecting other related signaling pathways.

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Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor

  • 实验方案

  • 发表日期：

    2025年

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  • Aqfan Jamaluddin

摘要

This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET). Focusing on the eBRET2 and NanoBRET™ variants, the chapter covers steps from cell culture to transfection, ligand stimulation, and BRET measurements, offering a robust protocol to examine the temporal aspects of Gq signaling in live cells. This methodology facilitates a nuanced understanding of Gq-mediated cellular responses, but also provides researchers with a valuable tool for dissecting other related signaling pathways.