Scholarlink

This chapter presents a novel microscopy technique, real-time ultra-large scale imaging with high-resolution microscopy (RUSH), which enables imaging of biological systems with unprecedented space–bandwidth product and data throughput. We describe the design and implementation of the RUSH system, which comprises a customized objective lens, a camera array, and a computer cluster. We also demonstrate the applications of the RUSH system in imaging neural activity in mouse brains and other biological samples with curved surfaces. Further, we introduce algorithms for image denoising and neural signal detection, and discuss the challenges and opportunities for large-scale imaging. The chapter aims to provide readers with an overview of the RUSH system and its potential for advancing system biology studies.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Real-Time Ultra-Large-Scale Imaging with High-Resolution Microscopy

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Qionghai Dai,
  • Hao Xie,
  • Jiamin Wu

摘要

This chapter presents a novel microscopy technique, real-time ultra-large scale imaging with high-resolution microscopy (RUSH), which enables imaging of biological systems with unprecedented space–bandwidth product and data throughput. We describe the design and implementation of the RUSH system, which comprises a customized objective lens, a camera array, and a computer cluster. We also demonstrate the applications of the RUSH system in imaging neural activity in mouse brains and other biological samples with curved surfaces. Further, we introduce algorithms for image denoising and neural signal detection, and discuss the challenges and opportunities for large-scale imaging. The chapter aims to provide readers with an overview of the RUSH system and its potential for advancing system biology studies.