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We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium, Cell growth SFM Medium, is a serum-free medium designed to maintain cell growth with high-viability profiles. The second corresponds to a protein-free version optimized to increase CHO recombinant protein production (Production PFM Medium). Considerations on the use of these formulations for other cell culture assays (such as transfections) are also described.

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Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines

  • 实验方案

  • 发表日期：

    2025年

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  • Berta Capella Roca,
  • Justine Meiller,
  • Padraig Doolan,
  • Martin Clynes

摘要

We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium, Cell growth SFM Medium, is a serum-free medium designed to maintain cell growth with high-viability profiles. The second corresponds to a protein-free version optimized to increase CHO recombinant protein production (Production PFM Medium). Considerations on the use of these formulations for other cell culture assays (such as transfections) are also described.