Biosorption of Heavy Metals by Lactic Acid Bacteria for Detoxification

The gradual accumulation of heavy metals can have detrimental effects on health. Lactic acid bacteria (LAB) are common microbes used as probiotics; various LAB strains are consumed in food products, especially in fermented foods. Many studies have suggested that LAB with high affinity to harmful heavy metals can be used as efficient detoxification tools. Accordingly, it is important to test the biosorption of various heavy metals, e.g., cadmium, lead, arsenic, and mercury, by LAB. Here, I describe protocols to quantify the binding ability of LAB and to identify their heavy metal-binding proteins.