MRI is a non-invasive and cost-effective technique that uses magnetic fields to create images of the brain. Structural MRI is based on the fact that biological tissues have high water content. MR images provide high-resolution anatomical information with great contrast of both the gray matter (GM) and the white matter (WM). Advanced imaging and analysis techniques have been successfully developed to gain insights into the pathophysiology of PD, including its diagnosis, progression, symptoms, and treatment effects. Structural MRI, which focuses on the anatomical features of the brain, is particularly useful in studying PD. There are different methods for analyzing structural MRI in PD, including voxel-based and surface-based approaches. This chapter presents the clinical and pathophysiological rationale for the use of MRI in PD and introduces novel and established techniques that contributed to a better understanding of PD and its impact of the spread of the pathology to distinct CNS regions.