Ex Vivo Digestion with Human Gastrointestinal Juices
摘要
Utilization of human gastrointestinal juices in model digestion presents a promising approach for simulating human digestion of food matrixes in a highly relevant and physiological manner. This approach offers advantages in terms of gastrointestinal enzyme specificity, that more closely mimics human physiology. In this protocol, we outline the process of aspirating gastrointestinal juices, conducting enzyme assays, and performing ex vivo model digestions. Additionally, we supply examples of foods and matrixes where the use of human gastric and duodenal juices has significantly enhanced the authenticity and utility of digestion studies.