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Red light penetrates deep into mammalian tissues and has low phototoxicity. We developed a red light–activatable photoswitch (MagRed) for deep tissue optogenetics. Using MagRed, we developed a red light–activatable endogenous gene transcription system (Red-CPTS) based on CRISPR–Cas9. Here we provide a detailed protocol for endogenous gene activation using Red-CPTS in cultured mammalian cells and living mice in vivo.

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A Red Light–Activatable Endogenous Gene Transcription System with Red-CPTS

  • 实验方案

  • 发表日期：

    2025年

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  • Takahiro Nakajima,
  • Yuto Kuwasaki,
  • Shota Yamamoto,
  • Takahiro Otabe,
  • Moritoshi Sato

摘要

Red light penetrates deep into mammalian tissues and has low phototoxicity. We developed a red light–activatable photoswitch (MagRed) for deep tissue optogenetics. Using MagRed, we developed a red light–activatable endogenous gene transcription system (Red-CPTS) based on CRISPR–Cas9. Here we provide a detailed protocol for endogenous gene activation using Red-CPTS in cultured mammalian cells and living mice in vivo.