Transplant and Care for In Vitro Orchid Seedlings

Transferring young orchid seedlings from in vitro culture to the external environment is critical for orchid micropropagation. This chapter introduces the techniques for transitioning orchid seedlings from tissue culture to greenhouse conditions. We highlight the diverse characteristics of orchids, including their various vegetative organs and ecological niches, which influence their care requirements. The chapter provides practical guidelines for deflasking orchid seedlings, preparing potting media, selecting pots, and using appropriate tools and chemicals. Methods for deflasking, potting, and general maintenance post-deflasking cover light intensity, watering, humidity, temperature, and fertilizing aspects are detailed. The information provides insights into identifying optimal deflasking timing and addressing common challenges encountered during the process.