Characterization of Polyphenols-Protein Interactions: From Chemistry to Food Technology and Human Nutrition
摘要
This chapter presents an overview of the application of spectroscopic methodologies, such as fluorescence spectroscopy and saturation transfer difference nuclear magnetic resonance (STD-NMR), for the examination of complex molecular interactions involving proteins and polyphenols. Fluorescence spectroscopy provides valuable information regarding the dynamic aspects of binding kinetics and thermodynamics. On the other hand, STD-NMR supplies essential atomic-level details on the binding interfaces. The integration of these approaches enables a thorough investigation of the protein-polyphenol interaction landscape, thereby advancing our comprehension of their implications in the context of health and disease and expediting the progress of focused therapeutic interventions.