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Yeast Expression and Activity Assay of RVFV Polymerase

  • Xue Wang,
  • Lili Tang,
  • Eiling Chen,
  • Zhongzhou Chen

摘要

Rift Valley fever (RVF) virus is widespread worldwide and poses a severe threat to human life and property. RVF viral polymerase plays a vital role in the replication and transcription of the virus. Here, we describe how to express and purify this polymerase and perform tests for its in vitro activity assays.