Single-Cell Sequencing of 3′ RNA Transcripts

Single-cell RNA sequencing (scRNA-seq) enables the measurement of RNA expressed from individual cells within a tissue or population. RNA expression profiles may be used to draw conclusions about cellular states, cell subtypes within the population, responses to perturbations, and cellular behavior in the context of disease. Here we describe a method for scRNA-seq via single-cell encapsulation and capture of the polyadenosine tails at the 3′ end of mRNA transcripts combined with cell and molecular barcoding, allowing for the sequencing of 3′ untranslated regions in order to identify expressed genes from a cell.