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Quantitative Analysis of the Inactivation Process of Internalized Bacteria in Dictyostelium Cells

  • Lou W. Kim,
  • Victor Osorio-Castillo

摘要

The understanding of the inactivation process of ingested bacteria by phagocytes is a key focus in the field of host-pathogen interactions. Dictyostelium is a model organism that has been at the forefront of uncovering the mechanisms underlying this type of interaction. In this study, we describe an assay designed to measure the inactivation of Klebsiella aerogenes in the phagosomes of Dictyostelium discoideum.