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Carotenoids and Tocopherol Profiling in Fleshy Fruits

  • Bruno Silvestre Lira,
  • Aline Bertinatto Cruz,
  • Magdalena Rossi,
  • Luciano Freschi

摘要

Carotenoids and tocopherols are among the most powerful lipophilic antioxidants accumulated in fruit and vegetable crops. This chapter describes a method for the separation and quantification of carotenoids/chlorophylls and tocopherols based on microextraction followed by reverse- and normal-phase HPLC, respectively. Using this method, high-throughput, accurate analysis of these compounds can be performed in leaf and fruit samples.