Total Mercury Determination in Fish Muscle: Direct Analysis by Atomic Absorption Spectrophotometer

Mercury, a toxic heavy metal, has the potential to accumulate in humans, particularly in vulnerable groups such as pregnant women and children, posing significant health risks. Accurate assessment of mercury in food is crucial to ensure the absence of this contaminant or that intake is below the maximum permitted limits. Absorption Spectrometry (AAS) is an effective method for detecting trace levels of mercury in food, and it is recognized by international regulatory bodies. Despite advantages, common mercury analysis methods have limitations due to challenges associated with sample digestion. The technique based on direct mercury analysis by atomic absorption with thermal decomposition and amalgamation, without the need for prior sample preparation, offers significant benefits. Direct AAS plays a crucial role in food safety regulations by quantifying mercury content accurately, which is vital for consumer protection. In this chapter, we present a streamlined and rapid protocol for quantifying mercury levels in fish muscle through direct analysis using an atomic absorption spectrophotometer.