A Rapid Method for Screening Microplastic in Seafood Using Nile Red (NR) and Fluorescence Preceding Spectroscopic Polymer Analysis
摘要
Microplastic analysis in seafood is challenging in terms of size, quality assurance, and time-consuming efforts. In this chapter, we describe a rapid and simple method for analyzing microplastics (0.02–5 mm) in seafood tissues. The procedure involves alkaline digestion, a single filtration step, and further visual screening of potential microplastics by means of nile red staining and analysis under stereo microscope using a fluorescent lamp (450 nm excitation – 500 nm emission) as an initial step preceding the spectroscopic identification of polymer structure.