Determination of N-nitro-, C-nitro-, and C-nitrous-type Compounds in Meat Products by d-SPE Extraction and Liquid Chromatography Coupled to Mass Spectrometry
摘要
This method is intended for the extraction of 2-methyl-1,4-dinitropyrrol (DNMP), 1-nitro-2-methyl-3-amino pyrrole (NMAP), and ethylnitrolic acid (ENA) from meat products and analysis by reversed-phase liquid chromatography (with C18 as stationary phase) coupled to tandem mass spectrometry with electrospray ionization source (LC-ESI-MS/S). The analytes quantitation is performed by matrix-matched analytical curves and internal standard calibration with deuterated N-nitrosopyrrolidine (NPYR-d4).