Immunostaining for Epigenetic Modifications in Fagopyrum Calli
摘要
Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single target protein. It is commonly used in research and provides information about cellular localization and protein expression levels. This chapter describes a detailed protocol for immunostaining fixed Fagopyrum calli embedded in Steedman’s wax using nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines and DNA methylation.