Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid Overlay Technique
摘要
Compared to two-dimensional monolayer culture, cells cultured in three-dimensional (3D) platforms provide a more biochemically and physiologically relevant environment to study cell–cell and cell–extracellular matrix interactions in vitro. Using the liquid overlay technique, a scaffold-free method to generate 3D spheroids from human adipose-derived stem cells is described.