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Isolation of Human Adipose-Derived Stem Cells

  • Elizabeth Montano,
  • Sara Al-Ghadban,
  • Bruce A. Bunnell

摘要

Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine. We present the protocols from our laboratory for isolating and expanding hASCs. The isolation of hASCs involves the enzymatic digestion of adipose tissue and subsequent culturing of the isolated cells.