Quantification of Secondary Lipid Oxidation Products in Oils, Fats, and Food Products: Malondialdehyde by HPLC-DAD and TBA-RS by UV-Visible Spectrophotometry

This chapter presents an accurate method to prepare, analyze, and quantify secondary lipid oxidation products namely malonaldehyde MDA (as MDA-(TBA)2) and TBA reactive substances (TBA-RS). Each step of the extraction of the targeted compounds, preparation of MDA standard, external calibration, and finally analysis of the reaction media are presented. For the latest, we present two alternative procedures: the first is HPLC paired with a diode array detector (DAD) to specifically quantify MDA and the second is UV-visible spectrophotometry for the quantification of TBA-RS. The limit of quantification is 0.25 μM (250 nM) or 1 nmoles eq MDA g−1 lipids.