Abundance Calculation of Mycophagous Amoebae

Mycophagous protists occur in most soils, but their proper counting methods are lacking. As reported in the literature, mycophagous amoebae can be identified using fungal spores as bait where one needs to culture the mycophagous amoebae with the fungal spores and incubate for several days. In this chapter, two methods are mentioned; one is direct cultivation of amoebae with the spores to find out the lysed and perforated spores, and the other is 24-well microtiter-based method which is known as most probable number (MPN) method.