Unequivocal Identification Through Fluorescence In Situ Hybridization (FISH) Technique
摘要
Protists represent the largest portion of the diversity of eukaryotes in the globe and have been recognized for performing indispensable activities in biogeochemical cycles. With the advancement of molecular biology techniques, this group of living beings has been widely studied. Among molecular techniques, FISH assay stands out, which, through the hybridization of specific probes, provides an unequivocal phylogenetic relationship. Hence here we present the FISH assay, indicating step by step how to successfully perform the in situ hybridization process.