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Classical Wet Mount Method for Observing Live Protists

  • Komal A. Chandarana,
  • Natarajan Amaresan

摘要

Protist ecology has used optical microscopes for observing cell features since very beginning. A dissecting microscope with light- and dark-field illumination is ideal for observing protists. The basic method adopted for observing live protists under the microscope is classical wet mount method. Though it is a very primitive method, it is an essential part of the protistological studies till today for observing live cell morphology, locomotion, and its life stage (trophozoites or cyst).