Cryptococcus neoformans Transcriptome Analysis to Identify Differentially Expressed Genes Using the STAR Pipeline in CyVerse Discovery Environment
摘要
RNA sequencing is a next-generation sequencing approach that may be used to investigate many aspects of gene expression changes between cells. Analysis of the data is typically a multistep process using several bioinformatics tools. The following protocol utilizes a reliable pipeline for identifying differentially expressed genes among samples of Cryptococcus neoformans that is approachable for the adventurous beginner.