Analysis of Cryptococcus Extracellular Vesicles
摘要
Extracellular vesicles (EVs)Extracellular vesicles (EVs) are produced by all domains of life. In fungal pathogens, they participate in virulenceVirulence mechanisms and/or induce protective immunity, depending on the pathogenic species. EVs produced by pathogenic members of the Cryptococcus genus mediate virulenceVirulence, antifungal resistance, as well as humoral and cell-mediated immunity. The isolation of cryptococcal EVs has been laborious and time-consuming for years. In this chapter, we detail a fast protocol for the isolation and analysis of EVs produced by members of the Cryptococcus genus.