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Analysis of the Snake Venom Peptidome

  • Solange M. T. Serrano,
  • André Zelanis,
  • Jackson G. Miyamoto,
  • Jackelinne Y. Hayashi,
  • Eduardo S. Kitano,
  • Alexandre K. Tashima

摘要

Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.