A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. Stereological methods are nowadays an essential tool to quantify cells or other types of structures in an unbiased and reproducible manner. In this chapter, a general protocol for a stereological estimation of the relative volume density of each structural component (Vv), which can be applied to any organ/structural component, will be described.