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Microbial Permeation Through Food Packaging Materials

  • Julia V. Ernesto,
  • Patricia Severino,
  • Anna C. Venturini,
  • Cristiana M. P. Yoshida,
  • Classius F. da Silva,
  • Patricia S. Lopes

摘要

Microbial permeation is an essential property in developing food packaging materials. Here we describe a simple method to determine it by placing the film on open vials containing nutrient broth. Negative and positive vials are also provided in this method. The tested vials are placed in an open environment for about ten days. The turbidity of the nutrient broth in any vial is recorded as microbial contamination, meaning the microorganism could permeate through the packaging material. This straightforward protocol can be invoked by material developers targeting microbial-tight packaging.