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Unraveling Physical Interactions of Clostridioides difficile with Phage and Phage-Derived Proteins Using In Vitro and Whole-Cell Assays

  • Wichuda Phothichaisri,
  • Tanaporn Phetruen,
  • Surang Chankhamhaengdecha,
  • Tavan Janvilisri,
  • Puey Ounjai,
  • Robert P. Fagan,
  • Sittinan Chanarat

摘要

Physical interactions between bacteria and phages provide valuable insights into the mechanisms of phage infection and may provide information on the use of phages as a therapeutic approach. In this study, we employed a combination of in vitro and whole-cell assays to examine the interactions between Clostridioides difficile and phages and phage-derived proteins. These techniques can also be adapted for studying the physical interactions between other bacterial species and their associated phages.