Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging

实验方案
发表日期：

2024年

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Stefanie Kuhns,
Alice Dupont Juhl,
Zeinab Anvarian,
Daniel Wüstner,
Lotte B. Pedersen,
Jens S. Andersen

摘要

CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.