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Measurement of Ice Nucleation Activity of Biological Samples

  • Rosemary J. Eufemio,
  • Ralph Schwidetzky,
  • Konrad Meister

摘要

Experimentation with ice-nucleating biomolecules is needed to advance the fundamental understanding of biotic heterogeneous ice nucleation. Standard experimental procedures vary with sample type. Here we describe a generalized primary purification and analysis process to measure ice nucleation activity of biological samples using an advanced freezing droplet assay.