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Begomovirus Inoculation in Arabidopsis and Cassava

  • José T. Ascencio-Ibáñez,
  • Mary M. Dallas,
  • Linda Hanley-Bowdoin

摘要

The use of infectious clones to inoculate plant viruses allows for controlled studies that lead to a better understanding of plant–virus interactions. The main methods used for laboratory inoculation of geminiviruses are agroinoculation and biolistics. We describe how to successfully inoculate geminiviruses, focusing on Arabidopsis as a model plant and cassava as a crop.